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Fityk peak seperation7/28/2023 ![]() B) Inclusion complex formationīearing the above in mind, our first step was to test the efficiency of many types of CDs to encapsulate MeJA 6. It is a very useful technique for separating compounds and, if we introduce CDs too at the same time as the MeJA mixture, the four stereoisomers will be better separated. HPLC can be thought of a 400 meters hurdles race, in which the best-trained athlete will win the race. 2), but can separate isomer compounds using their strong affinity for each other. CDs can not only complex hydrophobic compounds to form inclusion complexes ( Fig. To do this, we used cyclodextrins (CDs, doughnut-shaped oligosaccharides made up of α-linked glucose units) in the mobile phase of the HPLC (high-performance liquid chromatography). However, these methods are expensive and difficult to carry out so our research group 5 successfully separated and identified all four stereoisomers using a simple and sensitive HPLC-RP method. Several methods have been created to obtain the four stereoisomers: asymmetric synthesis, enzymatic resolution and HPLC resolution prior to diastereoisomer formation 4. This is important because, if we used one of the above, we would obtain a higher concentration of the bioactive compounds of interest without mixing it with other isomers. 1): (+)epiMeJA, (-)epiMeJA, (+)MeJA and (-)MeJA and when the effect of post-harvest treatment with stereoisomers of MeJA on the bioformation of myricetin, quercetin and kaempferol was studied in red raspberry, the results obtained depended on the stereoisomer used 3. Nevertheless, the commercial MeJA is a mixture of the four stereoisomers ( Fig. ![]() Structure of the four stereoisomers of MeJA 1) (this molecule is involved in the production of some bioactive compounds both in plants and humans, for example, resveratrol 2, Taxol® etc. In this case, we shall focus on one molecule, Methyl Jasmonate (MeJA, Fig. ![]() One way to do this is to increase the yield using a technique called elicitation, which involves using certain molecules or conditions to enhance the production of a given bioactive compound in plant cell cultures 1. In recent years, plants have been studied to find new products, and furthermore, many researchers have focused on improving the production of bioactive secondary metabolites in plants. The industry also knows that these things are necessary, which is why they have developed a huge range of products, increasing interest in finding new ingredients with high added value, for example. This is because current society demands the same things as Burr society: good food, free time with family and friends, and the more healthy you are and longer you live, the better. (1756 –1836), the Vice-President of the United States under Thomas Jefferson said something that is still applicable today: “The rule of my life is to make business a pleasure, and pleasure my business”. Author: Adrián Matencio is pursuing a PhD at the Department of Biochemistry and Molecular Biology – A, Faculty of Biology, University of Murcia, where he graduated in biotechnology.Ī long time ago, Aaron Burr Jr. ![]()
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